Study of Polymorphism of the DRD2 Gene (-141C Ins/Del, rs1799732) with Attention Deficit Hyperactivity Disorder a Population Sample of Children in Iranian-Azeri

Mehdizadeh Fanid, Leila; Adampourezare, Mina; HosseinpourFeizi, Mohammad Ali; Noorazar, SeyedGholamreza

doi:10.22038/ijp.2016.21049.1764

Study of Polymorphism of the DRD2 Gene (-141C Ins/Del, rs1799732) with Attention Deficit Hyperactivity Disorder a Population Sample of Children in Iranian-Azeri

Authors

Leila Mehdizadeh Fanid ¹

Mina Adampourezare ²

Mohammad Ali HosseinpourFeizi ³

SeyedGholamreza Noorazar ⁴

¹ Cognitive Neuroscience, PhD. Department of Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.

² Physiology, MSc. Department of Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.

³ Radiobiology, Prof. Department of Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran.

⁴ Child and Adolescent Psychiatrist MD & Psychiatry assistant professor of Tabriz university of medical science, Tabriz, Iran.

10.22038/ijp.2016.21049.1764

Abstract

Background
Attention deficit hyperactivity disorder (ADHD), is a multifactorial disorder and converging evidence has implicated abnormalities of dopamine neurotransmission. The aim of this study was to examine the association of -141 polymorphisms in DRD₂ gene with ADHA among Iranian-Azeri population.
Materials and Methods
A case–control association study included 153 patients with attention deficit hyper activity disorder (case group), and 133 healthy subjects (control group). Genomic DNA was extracted peripheral blood samples by salting-out method. Single nucleotide polymorphism (SNP) genotyping was performed by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The data analysis was performed through Chi-square, with a significance level of 0.05.
Results: There was not significant difference in the allele and genotype frequencies between ADHD and -141C Ins/Del polymorphism in cases and controls (P>0.05). Ins/Ins homozygous dominants were more frequent in control group than the case group, but there was not significant difference observed (P>0.05). Del/Del homozygous dominants were not observed. No significant difference was detected in the allele and genotype frequencies between ADHD and -141 Insertion/Deletion polymorphism in cases and control groups (P>0.05).
Conclusion
Our results do not detected association between the -141C Ins/Del, rs1799732, polymorphism and ADHD disorder in population of Children in Iranian-Azeri.

Keywords

ADHD

DRD2 gene

Iranian children

Polymorphism