Document Type : original article
Authors
1 MScD, Associate Professor of Pediatric Department, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran
2 Dentist (DDS), School of Dentistry, Islamic Azad University of Isfahan (Khorasgan) Branch, Isfahan, Iran.
3 Dentist (DDS), School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.
4 PhD candidate, Department of Biostatistics, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract
Background: salivary proteins have, today, gained special importance in studies of the role of saliva in tooth decay. Among the peptides, histatins and defenses play a more important role. The aim of this study was to determine the concentration of salivary antibacterial peptides in children with early childhood caries (ECC, SECC) compared to children without caries.
Methods: This comparative-case study was conducted on 48 young children (under 6 years of age) with milk teeth referred to a private pediatric dentistry center. The participants were divided into three groups of 16 with early childhood caries (ECC, SECC) and without decay. After collecting children's saliva, the samples were sent to the laboratory to obtain peptide concentration in salivia using ELISA and the results were analyzed using SPSS 22 software.
Results: The average index of histatin-5 in the mild caries group was significantly lower than those in the moderate caries (p<0.001) and severe caries groups (p<0.001). The average index of beta-defensin-1 in the mild caries group was significantly higher than those in the moderate caries (p<0.001) and severe caries groups (p<0.001). The average beta-defensin-2 index in the mild caries group was significantly higher than those in the moderate caries group (p<0.001); and in the severe caries group, it was significantly higher than that in the moderate caries group (p<0.001).
Conclusion: With the increase of salivary HST-5, the progress of caries increased. Also, the progress of caries was associated with a decrease in the amount of β-defensin-1. No correlation was observed between the caries process and the amount of β-defensin-2.
Keywords
- Barnet M, William L, Shafer G, Hine MK. Shafer’S Textbook of Oral Pathology (6Th Edition) - R. Rajendran - Google Books. In: Shafer’S Textbook of Oral Pathology. 2009. p. 412–3.
- Chu C, Mei ML, Lo ECM. Use of fluorides in dental caries management. Gen Dent. 2010; 58(1):37–43.
- Nematollahi H, Mehrabkhani M, Sheykhani M. Assessing the Relationship between Diet and Prevalence of Early Childhood Caries in Birjand Preschool Children. J Dent. 2007; 8(1):70–85.
- Meyer F, Enax J. Early Childhood Caries: Epidemiology, Aetiology, and Prevention. Vol. 2018, International Journal of Dentistry. Hindawi Limited; 2018.
- Pract AA-JCD, 2017 undefined. Early childhood caries: A review. thejcdp.com. 2017 Aug 1; 18(8):732–7.
- Subramanyam D, Gurunathan D, Gaayathri R, Priya VV. Comparative evaluation of salivary malondialdehyde levels as a marker of lipid peroxidation in early childhood caries. Eur J Dent. 2018 Jan 1; 12(1):67–70.
7. Lenka S, Swain SK, Bhuyan R SM. Fungal infection in the oral cavity: A review. Int J Cur Res Rev. 2020;
12(18):149–53.
- Oudhoff MJ, Van Den Keijbus PAM, Kroeze KL, Nazmi K, Gibbs S, Bolscher JGM, Veerman ECI. Histatins enhance wound closure with oral and non-oral cells. J Dent Res. 2009 Sep 18; 88(9):846–50.
- Rudney JD, Staikov RK, Johnson JD. Potential biomarkers of human salivary function: A modified proteomic approach. Arch Oral Biol. 2009 Jan 1; 54(1):91–100.
- Cirioni O, Giacometti A, Ghiselli R, Orlando F, Kamysz W, D’Amato G, Mocchegiani F, Lukasiak J, Silvestri C, Saba V, Scalise G. Potential therapeutic role of histatin derivative P-113D in experimental rat models of Pseudomonas aeruginosa sepsis. J Infect Dis. 2004; 190(2):356–64.
- Helmerhorst EJ, Alagl AS, Siqueira WL, Oppenheim FG. Oral fluid proteolytic effects on histatin 5 structure and function. Arch Oral Biol. 2006 Dec 1; 51(12):1061–70.
- Varkey J NR. Antibacterial activity of human neutrophil defensin HNP-1 analogs without cysteines. Antimicrob Agents Chemother. 2005; 49(11):4561–6.
- Dowling P, Wormald R, Meleady P, Henry M, Curran A, Clynes M. Analysis of the saliva proteome from patients with head and neck squamous cell carcinoma reveals differences in abundance levels of proteins associated with tumor progression and metastasis. J Proteomics. 2008 Jul 21;71(2):168–75.
- Amerikova M, Pencheva El-Tibi I, Maslarska V, Bozhanov S TK. Antimicrobial activity, mechanism of action, and methods for stabilization of defensins as new therapeutic agents. Biotechnol Biotechnol Equip. 2019; 33(1):671–82.
- Sahl HG, Pag U, Bonness S, Wagner S, Antcheva N TA. Mammalian defensins: structures and mechanism of antibiotic activity. J Leukoc Biol. 2005; 77(4):466–75.
- Piekoszewska-Ziętek P, Turska-Szybka A, Olczak-Kowalczyk D. Salivary proteins and peptides in the etiology of caries in children: Systematic literature review. Oral Dis. 2019 May 1; 25(4):1048–56.
17. Munther S. The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity
in relation to nutritional status. Saudi Dent J. 2020 Feb 11; 32(8):410–6.
- Lips A, Antunes LS, Antunes LA, Abreu JGB De, Barreiros D, Oliveira DSB De, Batista AC, Nelson-Filho P, Silva LAB Da, Silva RAB Da, Alves GG, Küchler EC. Genetic Polymorphisms in DEFB1 and miRNA202 are involved in Salivary Human β-Defensin 1 Levels and Caries Experience in Children. Caries Res. 2017; 51(3):209–15.
- Prabakar J, Arumugham IM, Sakthi DS, Kumar RP, Leelavathi L. Prevalence and Comparison of Dental Caries experience among 5 to 12 year old school children of Chandigarh using dft/ DMFT and SiC Index: A Cross-sectional study. J Fam Med Prim Care. 2020; 9(2):819.
20. Gornowicz A, Tokajuk G, Bielawska A, Maciorkowska E, Jabłoński R, Wójcicka A, Bielawski K. The assessment of sIgA, histatin-5, and lactoperoxidase levels in saliva of adolescents with dental caries. Med Sci Monit. 2014; 20:1095–
100.
- Jurczak A, Kościelniak D, Papież M, Vyhouskaya P, Krzyściak W. A study on β-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression. Biol Res. 2015 Oct 31; 48.
22. Munther S. The impact of salivary lactoperoxidase and histatin-5 on early childhood caries severity in relation to nutritional status. Saudi Dent J. 2020;
32(8):410–6.
- Wu CC, Lin CT, Wu CY, Peng WS, Lee MJ, Tsai YC. Inhibitory effect of Lactobacillus salivarius on Streptococcus mutans biofilm formation. Mol Oral Microbiol. 2015 Feb 1; 30(1):16–26.
- Rodrigues Ribeiro T, Dria KJ, Barreto Mano Carvalho C DE, Jalles Monteiro A, Claudino Fonteles MS, Moraes Carvalho K DE, Fonteles R. Salivary peptide profile and its association with early childhood caries. Wiley Online Libr. 2012 May; 23(3):225–34.
- Tao R, Jurevic RJ, Coulton KK, Tsutsui MT, Roberts MC, Kimball JR, Wells N, Berndt J, Dale BA. Salivary antimicrobial peptide expression and dental caries experience in children. Antimicrob Agents Chemother. 2005 Sep; 49(9):3883–8.
- Phattarataratip E, Olson B, Broffitt B, Qian F, Brogden KA, Drake DR, Levy SM, Banas JA. Streptococcus mutans strains recovered from caries-active or caries-free individuals differ in sensitivity to host antimicrobial peptides. Mol Oral Microbiol. 2011 Jun; 26(3):187–99.
- Dale BA, Krisanaprakornkit S. Defensin antimicrobial peptides in the oral cavity. J Oral Pathol Med. 2001 Jul 1; 30(6):321–7.
- Krisanaprakornkit S, Janet R. Kimball, Aaron Weinberg, Richard P. Darveau, Brian W. Bainbridge BAD. Inducible Expression of Human β-Defensin 2 byFusobacterium nucleatum in Oral Epithelial Cells: Multiple Signaling Pathways and Role of Commensal Bacteria in Innate Immunity and the Epithelial Barrier. Infect Immun. 2000; 68(5):2907–15.
- Ouhara K, Komatsuzawa H, Yamada S, Shiba H, Fujiwara T, Ohara M, Sayama K, Hashimoto K, Kurihara H, Sugai M. Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, β-defensins and LL37, produced by human epithelial cells. J Antimicrob Chemother. 2005; 55(6):888–96.
- G Calado, I Behl, HJ Byrne FL. Raman spectroscopic characterisation of non-stimulated and stimulated human whole saliva. Clin Spectrosc. 2021; 1(3):100010.
- Chung WO, Dommisch H, Yin L DB. Expression of Defensins in Gingiva and Their Role in Periodontal Health and Disease. Curr Pharm Des. 2007; 13(30):3073–83.
- Guedes SFF, Neves BG, Bezerra DS, Souza GHMF, Lima-Neto ABM, Guedes MIF, Duarte S, Rodrigues LKA. Saliva proteomics from children with caries at different severity stages. Oral Dis. 2020 Sep 1; 26(6):1219–29.
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