Journal of Pediatric Perspectives

Stem Cells So far! Stem Cell Therapy Facts and Principles

Elahe Mahdipour

Volume 2, Issue 2.3 , May 2014, , Pages 5-5

https://doi.org/10.22038/ijp.2014.2654

Abstract

Stem cells are cells with the ability to divide for indefinite periods and to give rise to specialized cells. Stem cell therapy has now been emerged as an extraordinary promise ... Read More

A Review on Bioethics and Legal Concerns in Stem Cell Researches from Embryo Point of View

Farzad Mahmoodian; Bita Pakniyat Jahromi; Nazanin Setayeshpour

Volume 2, Issue 2.3 , May 2014, , Pages 31-31

https://doi.org/10.22038/ijp.2014.2684

Abstract

Introduction: Sharply progression and development in different aspect of technology and science in the field of medicine and sanitation bring many challenges in biological moral and ... Read More

Maxillary alveolar bone grafting: the role of Platelet-rich plasma (PRF)

Saeedeh Khajehahmadi

Volume 2, Issue 2.3 , May 2014, , Pages 32-32

https://doi.org/10.22038/ijp.2014.2685

Abstract

Introduction: Gold standard for alveolar cleft bone grafting is autogenous bone. Advantage of adding the growth factors to the bone is established, so adding PRF to the autogenous ... Read More

Stem Cells in Regenerative Endodontics

Maryam Forghani

Volume 2, Issue 2.3 , May 2014, , Pages 33-33

https://doi.org/10.22038/ijp.2014.2686

Abstract

Background Currently, clinical endodontics includes procedures that are based on the ability of stem cells to accomplish repair (eg, direct pulp capping, apexogenesis, apexification, ... Read More

Tracking Bone Marrow Mesenchymal Stem Cells Transplanted to Experimental Rabbit Model with Brain Trauma by MRI

Ayoob Rostamzadeh; Mohammad Jafar Rezaie; Reza Ahadi; Mohammad Farzizadeh; Rastegar Rahmani Tanha; Arash Shabani

Volume 2, Issue 2.3 , May 2014, , Pages 34-34

https://doi.org/10.22038/ijp.2014.2687

Abstract

Introduction Brain trauma is one of the most common causes of hospitalization, disability, and mortality of people aged 15 to 74 years in nowadays societies. On the other hand, nowadays, ... Read More Introduction Brain trauma is one of the most common causes of hospitalization, disability, and mortality of people aged 15 to 74 years in nowadays societies. On the other hand, nowadays, different categories of stem cells are used to treat different kinds of diseases. BM-MSCs, are center of scientific research because of their high differentiation and proliferation ability. Methods: Stem cells were isolated from animal bone marrow and after cultivation in passage 3, they were labeled with dose of 5 mg/ ml of USPIO (prepared by the Institute of Sharif Teb System), after 36 hours, they were incubated in the cultivation medium. To confirm the iron nanoparticles inside the cells Prussian blue staining was used. A number of 5 × 105 labeled MSC injected in rabbit the through the auricular artery , and the rabbits were imaged in coil wrists at time intervals of 1 week after injury , 1 ,3 ,10 ,16 and 24 days after injection by 1.5-T MRI system . In addition, Immunohistochemistry and RT-PCR studies were used to evaluate cells differentiation into neurons or neuroglia. Results: 24 hours after injection of MSCs, MRI images showed that almost half of the cells have accumulated in the lungs, however 72 hours later the labeled MSC signals were observed as hypointensive areas in the lesion site on the T2* relaxation time. Results also showed that 1.5 T MRI scanner is capable of tracking of the cells up to 16 days after injection. 8 animals at specified time intervals (up to day 16) were killed .The results of Immunohistochemistry and RT-PCR on day 64 after injection showed that the cells in the lesion site express the proteins, such as GFAP and Beta tubulin III, that are the markers of astrocytes and neurons, respectively. Conclusion: Furthermore, their differentiation into neuroglia cells or even neurons (without getting into the laboratory environment) could be a promising approach for treating different kinds of brain injuries and other disorders of the nervous system. However, another important issue is the restricting factor of the lung which traps a large number of systemically transplanted cells. Thus, the systemic injection through the artery is suggested. Keywords: Brain trauma, Cells transplantation, Mesenchymal stem cells (MSCs).

Operative Dentistry and Biomaterials for Tooth Regenration

Horieh Moosavi

Volume 2, Issue 2.3 , May 2014, , Pages 35-35

https://doi.org/10.22038/ijp.2014.2688

Abstract

Tissue-engineered biomaterials have existed approximately 40 years as simple biomimetic structures. Replacement of human tissue with new tissue can be accomplished by generating replacements ... Read More

Stem Cell in Breast Milk

Ahmadshah Farhat; Mohammadhasan Arjmand; Ashraf Mohammadzadeh

Volume 2, Issue 2.3 , May 2014, , Pages 36-36

https://doi.org/10.22038/ijp.2014.2689

Abstract

Tissue-engineered biomaterials have existed approximately 40 years as simple biomimetic structures. Replacement of human tissue with new tissue can be accomplished by generating replacements ... Read More

Tooth Regeneration with Stem Cell Sources

Neda Naghavi

Volume 2, Issue 2.3 , May 2014, , Pages 37-37

https://doi.org/10.22038/ijp.2014.2690

Abstract

Introduction: During the last decade, advances in tissue engineering and stem cell-based tooth regeneration have provided realistic and attractive means of replacing lost or damaged ... Read More Introduction: During the last decade, advances in tissue engineering and stem cell-based tooth regeneration have provided realistic and attractive means of replacing lost or damaged teeth. The first adult stem cells isolated from dental tissues were dental pulp stem cells (DPSCs). When transplanted with hydroxyl apatite/tri calcium phosphate (HA/TCP) powder, they formed a dentin-like structure lined with odontoblast-like cells that surrounded a pulp-like interstitial tissue. DPSCs could differentiate in vitro into other mesenchymal cell derivatives such as odontoblasts, adipocytes, chondrocytes, and osteoblasts and could also differentiate into functionally active neurons. Mesenchymal stem cells (MSCs) have also been isolated from the dental pulp of human deciduous teeth. When these cells are transplanted mixed with HA/TCP in vivo, they can form dentin and bone but not dentin–pulp complexes. Stem cells from the apical papilla (SCAPs) are found in the papilla tissue in the apical part of the roots of developing teeth. The third molars and teeth with open apices are an important source of SCAPs. Transplantation of SCAPs and periodontal ligament stem cells (PDLSCs) into tooth sockets allowed the formation of dentin and periodontal ligament. Dental follicle stem cells (DFSCs) have also been isolated from the follicles of developing third molars. They can differentiate into osteoblasts, adipocytes, and nerve-like cells in vitro and form cementum and periodontal ligament in vivo. Results: Future therapeutic approaches for the restoration of damaged dentin, pulp, cementum, and periodontal ligaments may make use of autologous stem cells that have been stored after removal from the patient. Conclusion:With regard to clinical application, these stem cells share the common obstacles of ethical concern arising from their embryonic origin, the risk of tumorigenesis, and the possibility of immune rejection after allogeneic transplantation. Keywords: Tooth Regeneration, Stem Cell.

Toxicity Study Of gelatin-chitosan Films with Bone Marrow Stromal Cells Cultured in Rat

Afsane Karami Juyani; Mehdi Saberi; Gholamreza Kaka; Mahvash Jafari; Simin Riyahi; Mahdieh Taghizadeh

Volume 2, Issue 2.3 , May 2014, , Pages 38-38

https://doi.org/10.22038/ijp.2014.2691

Abstract

Background :Gelatin and chitosan are known as biodegradable and biocompatible biopolymers.These biopolymers have recently receivedincreasingly more attention for tissueengineering.The ... Read More Background :Gelatin and chitosan are known as biodegradable and biocompatible biopolymers.These biopolymers have recently receivedincreasingly more attention for tissueengineering.The aim of this study was to evaluate the toxicity effects of gelatin-chitosan film on bone marrow stromal cell(BMSCs) culture in rat. Materials & Methods: First, gelatin- chitosan composites film were prepared by solution mixing, followed by film casting of both biopolymers in glacial acetic acid.After two passage of BMSCs culture, the cells were cultured in four plate groups including: control plates have no any film,gelatinplates,chitosan plates and gelatin- chitosan plates.Theproliferation,differentiation,viability and apoptosis rates of BMSCs were studied duringthesecond,fourthand sixth days.The activity of superoxidedismutase(SOD),catalase(CAT)and glutathione(GSH) and malondialdehyde(MDA) levels were determined by using biochemical methods. Results:The results showed that the mean BMSCs proliferation significantly reduced in chitosan group compared to control group (p<0.05),but in gelatin and gelatin- chitosan groups were similar to control group.Mean percentage of BMSCs apoptosis in all groups except chitosan group were similar to control group. Mean percentage of BMSCs viability at all groups was similar to control group except chitosan film. In addition, no significant changes were observed in CAT activity and GSH and MDA levels in comparison with control group.After72hours,SOD activity in gelatin-chitosan group was significantly reduced compared to other groups. No cell differentiation was detected in all groups. Conclusion: Results of proliferation, differentiation, apoptosis and antioxidantactivity in cultured BMSCs on a gelatin–chitosanfilm showed that gelatin-chitosanfilm can be used in tissue engineering and cell therapy as a good model of a biodegradable scaffold. Keywords: Antioxidant system, Bone Marrow StromalCells, Cell Proliferation andDifferentiation, Gelatin-Chitosan Film.

Human Mesenchymal Stem Cells Derived from Adiopose Tissue and Placenta and the Adipocytic and Osteocytic Differentiation

Zahra Mohammadi; Jalil Tavakol Afshari; Mohammad Reza Keramati; Daryoush Hamidi Alamdari; Meysam Ganjibakhsh; Azam Moradi Zarmehri; Ali Jangjoo; Mohammad Hadi Sadeghian

Volume 2, Issue 2.3 , May 2014, , Pages 39-39

https://doi.org/10.22038/ijp.2014.2692

Abstract

Introduction: Mesenchymal stem cells can be isolated from adult tissues, such as the adipose tissue, or other sources. Among all these sources, adipose tissue because of easy access, ... Read More

Study on Effect of Head, Tail, and Limbud extracts of Mouse on Differentiation of Hair Follicle Stem Cells to Neural cells

S Shojaie; R Khanbabaee; M Kasaian

Volume 2, Issue 2.3 , May 2014, , Pages 40-40

https://doi.org/10.22038/ijp.2014.2693

Abstract

Introduction: Adult stem cells are the group of cells which conserve their nature in tissues and organs among other cells. In recent years, the researchers reported the existence of ... Read More

Isolation, Characterization and Differentiation of Rat Adipose Tissue Derived Mesenchymal Stem Cells

Seyed Javad Hoseini; Hamed Ghazavi; Ahmad Ghorbani; Hamid Reza Sadeghian; Majid Ghayour Mobarhan; Elaheh Mahdipour

Volume 2, Issue 2.3 , May 2014, , Pages 41-41

https://doi.org/10.22038/ijp.2014.2694

Abstract

Introduction: Mesenchymal stem cells have the potential of self-renewal and differentiation into different cell types, including blood cells, heart, nerves and cartilage, ... Read More Introduction: Mesenchymal stem cells have the potential of self-renewal and differentiation into different cell types, including blood cells, heart, nerves and cartilage, and have unlimited power for division. These cells can be obtained from cord, before implantation from fertilized cells and also from various tissues of adults although the differentiation power and the ability to reproduce other cells are different. The use of these cells in the biological sciences, embryology and genetics are considered further.The aims of this study are extraction and characterization of stem cells with respect to differentiation of these cells into bone and fat cells in order to investigate the potential use of them in animal experimental models of stroke and heart attack and then in the case of the proper response at the bedside. Methods: An incision was made in the abdomen and about one gram of fat tissue was removed. After splitting under the hood, pieces of fat transferred into a sterile falcon tube and collagenase was added. The tube was put inside a water bath (37 ° C) for digestion fat components. After centrifugation the cells were extracted and cultured. After the third passage, the cells were placed in the appropriate ostogenic and adipogenic differentiation culture. To ensure the accuracy of the extraction method, the cell surface markers of the stem cells derived from human adipose tissue were analyzed by flowcytometry. Results: Staining with Oil Red and Alizarin Red showed that stem cells extracted by the aforementioned method have the ability to differentiate into bone and fat cells respectively. The results of flocytometry showed that CD34 and CD44 markers were negative and CD45 and CD105 were positive consistent with the immunological properties of Mesenchyma stem cells. Conclusions: This study showed that the mesenchymal stem cells derived from adipose tissue have the potential to differentiate into other cell types and due to the aims specified for continuation of this research, these cells can be used in the animal experimental models of stroke and myocardial infarction. Keywords: Adipose tissue, Mesenchymal stem cells,Rat.

Nanoscale Mechanical Stimulation of Human Mesenchymal Stem Cells

H Nikukar; S Reid; AS Curtis; MJ Dalby

Volume 2, Issue 2.3 , May 2014, , Pages 42-42

https://doi.org/10.22038/ijp.2014.2695

Abstract

Introduction: Mechanical stimulation of human mesenchymal stem cells has demonstrated changes in many cell behaviours such as adhesion, migration, growth and differentiation through ... Read More Introduction: Mechanical stimulation of human mesenchymal stem cells has demonstrated changes in many cell behaviours such as adhesion, migration, growth and differentiation through mechanotransductive pathways. These include experiments on effect of nanotopography 1, shear stress, stiffness of extracellular matrix 2, strain, stress and acoustic wave energy 3 on cells. In this research we were looking for mesenchymal stem cell responses to nanoscale mechanical vibrations in Z-axis. The changes in cell number and shape, differentiation and genetic changes were compared after stimulation with static control groups. Methods: A simple protocol for the stimulation of cells in nanoscale Z-axis has been developed for this project (Figure 1). Piezo actuator (type: P-010.00H by PI CeramicÒ) connected to the cell culture dish moves the entire surface up and down. The amount of displacement is dependent on the voltage applied. Attaching an aluminium disk to the base of the Petri dish ensures faithful transfer of the vibration to the cells. Human mesenchymal stem cells from bone marrow (PromoCellÒ) were seeded with 104 cells/dish. After 4 hours seeding and cell settlement, the cells were stimulated for 24 hours, 1 week, and 2 weeks. Experiments were performed in an incubator with optimal temperature 37°C and 5% CO2 concentration. The Petri dish was 60 mm x 15mm standard tissue culture treated polystyrene dish (52mm base diameter) from CorningÒ Incorporated and cell culture media used was MEM alpha modification with L-Glutamine and nucleosides from PAA laboratories (Austria) supplemented with 10% FBS and antibiotics (penicillin and streptomycin). Results: We observed significant responses after 1 and 2-week stimulations in cell number, cell shapes and phenotypical markers. Microarray was performed for all groups. Cell count showed normal cell growth with stimulation. However, cell surface area, cell perimeter, and arboration after 1-week stimulation showed significant increases. Immunofluorescent studies have showed significant increase in osteocalcin production after stimulation. Conclusions: Nanoscale mechanical vibration showed significant changes in human mesenchymal stem cell behaviours. Cell morphology changed to become more polygonal and increased expression of the osteoblast markers were noted. These findings with gene regulation changes suggesting nanoscale mechanostimulation has stimulated osteoblastogenesis. Keywords: Mesenchymal, Nanoscale, Stem Cells.

Effects of Inflammatory Cytokine Tumor Necrosis Factor-α on Human Mesenchymal Stem Cell Gene Expression: A Mechanism for Liver Regeneration

Maryam Ayatollahi; Ramin Yaghobi; Zeinab Sahraian; Rozita Zyaie

Volume 2, Issue 2.3 , May 2014, , Pages 43-43

https://doi.org/10.22038/ijp.2014.2696

Abstract

Introduction Insulin-like growth factor I (IGF-I) which is produced in abundance in the normal adult liver, is deeply involved in hepatocyte survival, growth, and differentiation ... Read More Introduction Insulin-like growth factor I (IGF-I) which is produced in abundance in the normal adult liver, is deeply involved in hepatocyte survival, growth, and differentiation during liver development. IGF-I plays the roles via the receptor (IGF-IR) signaling pathway. IGF-IR unlike IGF-I is expressed strongly in the developing liver, but much more weakly in adults. Objective: We hypothesized that inflammatory cytokines in liver injury may activate human liver progenitor cells to increase expression of IGF-1R, mediate IGF-1 and its cognate receptor-signaling pathways which contribute to liver regeneration. Methods: To study this, bone marrow-derived mesenchymal stem cells (MSCs) were aspirated from human normal donor after obtaining informed consent. Cells were divided into nine experimental groups and stimulated with inflammatory cytokine tumor necrosis factor-α (TNF-α). Evaluation of differential expression of IGF-1R in TNF-α-treated human MSCs was done by real time polymerase chain reaction. Results: The MSCs were CD11b (CR3), CD45, CD34, CD31 (PCAM-1), CD40, CD80 (B7-1), and HLA-class II negative because antigen expression was less than 5%, while they showed a high expression of CD90, and CD73. The differentiation of osteoblasts, is determined by deposition of a mineralized extracellular matrix in the culture plates that can be detected with Alizarin Red. Adipocytes are easily identified by their morphology and staining with Oil Red. The real time-PCR data reflected increased expression of IGF-IR in human MSCs upon TNF-α stimulation in a dose response manner. Conclusion: We concluded that ex vivo TNF-α affects IGF-1R expression in human marrow-derived MSCs and might contribute to in vivo-stimulation of liver progenitor cell affected by local inflammatory cytokines due to IGF-1R expression and liver regeneration after injury. It can be a strategy to improve stem cell-effectiveness before in vivo-cell transplantation. Keywords: Adult stem cells, Insulin-like growth factor-I, Gene expression, Liver regeneration.

Isolation, Culture, and Characterization of Human Dental Pulp Mesenchymal Stem Cells

P Mahdiyar; Sh Zare; R Robati; M Dianatpour; K Torabi; AD Tamadon; I Razeghian Jahromi2; A Tamadon; D Mehrabani

Volume 2, Issue 2.3 , May 2014, , Pages 44-44

https://doi.org/10.22038/ijp.2014.2697

Abstract

Introduction Based on previous researches, dental pulp stem cells (DPSCs) are easily accessible with limited morbidity after collection. Their embryonic origin, from neural crests, ... Read More Introduction Based on previous researches, dental pulp stem cells (DPSCs) are easily accessible with limited morbidity after collection. Their embryonic origin, from neural crests, explains their multipotency. DPSCs are primarily derived from the pulp tissues of the teeth.Objective: This study was undertaken to isolate, culture, and characterize two different third molar and first premolar human dental pulp mesenchymal stem cells. Methods: To obtain DPSCs, pulp tissues were removed from human third molar and first premolar teeth. They were digested by treating with collagenase type I. The extracted cells were passaged from primary culture up to passage 8. To enumerate the cells, the specified number of the cells were seeded into 24-well culture plates and the number of cells were counted to determine the growth curves of isolated cells from both type of teeth and the population duplication time (PDT) was determined. PCR and karyotype assays were performed to determine the cell surface mesenchymal markers and demonstrate the genetic stability of DPSCs, respectively. Results: The human DPSCs from both the third molar and the first premolar teeth were spindle-shaped in morphology. As growth curves showed, the proliferation rate of DPSCs in passage 8 among both teeth was different denoting to an increase in doubling time in the first premolar when compared to the third molar teeth. Normal karyotype of DPSCs derived from both the third molar and the first premolar teeth were exhibited. The isolated dental pulp stem cells expressed mesenchymal stem cell surface antigen. These cells were positive for CD73 but were negative for CD45 (hematopoietic stem cell marker). Conclusion: DPSCs can be an attractive candidate in regenerative medicine. As growth curves revealed, the first premolar teeth are suggested as a better source of MSC isolation. Keywords: Dental pulp, Growth curve, Mesenchymal stem cell, Molar tooth, Premolar tooth.

Platelet-rich plasma as filling material in open sinus lift surgery

Amin Rahpeyma

Volume 2, Issue 2.3 , May 2014, , Pages 45-45

https://doi.org/10.22038/ijp.2014.2698

Abstract

Background Many biomaterials are used during the open sinus lift surgery, beneath the elevated scheniderian membrane and maxillary sinus bony floor but the search for Ideal material ... Read More

Cancer stem cells: therapeutic targets

Mahdi Habibi-Anbouhi

Volume 2, Issue 2.3 , May 2014, , Pages 46-46

https://doi.org/10.22038/ijp.2014.2699

Abstract

Cancer stem cells (CSCs) have been identified as rare cellular populations in many cancers, including leukemia and solid tumors. This minor subpopulation of cancerous cells is immortal ... Read More

The characterization of CD marker profile of breast milk-derived stem cell

Mahsa Sani; Seyed Mojtaba Hoseini; Fatemeh Aleahmad; Mahin Salman Nejad; Sepideh Ebrahimi; Samira Jahanshahi; Tahereh Talaei

Volume 2, Issue 2.3 , May 2014, , Pages 47-47

https://doi.org/10.22038/ijp.2014.2700

Abstract

Background The mammary gland in humans is a dynamic organ that undergoes significant developmental changes during pregnancy, lactation, and involution. Stem cells derived from ... Read More

Osteoporosis after stem cell transplantation

Maryam Khalesi; Mehran Beiraghi Toosi

Volume 2, Issue 2.3 , May 2014, , Pages 48-48

https://doi.org/10.22038/ijp.2014.2701

Abstract

Background Stem cell transplantation has become as a novel treatment for end-stage kidney, lung, heart , liver diseases and several hematologic disorders. Improved survival of ... Read More

Review of Effect of Storage Time before Freezing on Stem Cells Surveillance, Collected from Cord Blood, Peripheral Blood and Bone Marrow

M Amirpour

Volume 2, Issue 2.3 , May 2014, , Pages 49-49

https://doi.org/10.22038/ijp.2014.2702

Abstract

Introduction: The use of cord stem cells, as a cell source for bone marrow reconstruction in patient under hematopoietic stem cell transplantation is enhancing. The amount of CD34+cells ... Read More

Evaluation of Stem Cell Markers, CD44/CD24 in Breast Cancer Cell Lines

Masoud Hashemi Arabi; Siamak Salami

Volume 2, Issue 2.3 , May 2014, , Pages 50-50

https://doi.org/10.22038/ijp.2014.2703

Abstract

Background Cancer stem cells play crucial roles in resistance to therapeutic schemes and relapse of disease, so it is important to find targeted therapies that kill them selectively. ... Read More

Wisdom Tooth as the Source of Stem Cells

Saeedeh khajehahmadi

Volume 2, Issue 2.3 , May 2014, , Pages 51-51

https://doi.org/10.22038/ijp.2014.2714

Abstract

Background Wisdom teeth are usually removed as a routine procedure for therapeutic or prophylactic reasons. Developing wisdom teeth are easy accessible source of stem cells during ... Read More

Ectopic Expression of Embryo/Cancer Sequence A (ECSA) in KYSE-30 Cell Line Using Retroviral System

M Khaleghizadeh; MM Forghanifard; M Gholamin; B Memar; MR Abbaszadegan

Volume 2, Issue 2.3 , May 2014, , Pages 52-52

https://doi.org/10.22038/ijp.2014.2715

Abstract

Background Human preimplantation embryonic cells share many similarities with cancer cells such as ability to self-renew, unlimited proliferation and maintenance of the undifferentiated ... Read More

Isolation of Adipose Tissue Stem Cells with Organ Culture Method

Ahmad Ghorbani; Seyed Amir Jalali; Masoumeh Varedi

Volume 2, Issue 2.3 , May 2014, , Pages 53-53

https://doi.org/10.22038/ijp.2014.2716

Abstract

Background Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results ... Read More

Advanced tissue engineering in periodontal Regeneration

Seyed Ali Banihashemrad

Volume 2, Issue 2.3 , May 2014, , Pages 54-54

https://doi.org/10.22038/ijp.2014.2717

Abstract

The old wishes of people were to regenerate lost tissues of periodontium that this fact is achieved by gen and cell therapy .Periodontal disease is a chronic inflammation around the ... Read More

Volume 12 (2024)

Volume 11 (2023)

Volume 10 (2022)

Volume 9 (2021)

Volume 8 (2020)

Volume 7 (2019)

Volume 6 (2018)

Volume 5 (2017)

Volume 4 (2016)

Volume 3 (2015)

Volume 2 (2014)

Volume 1 (2013)

Keywords = poster presentation

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract